Distributions of patient results from Fürst Medical Laboratory (March 2004) compared to NORIP distributions of reference values
Fürst Medical Laboratory, Oslo, Norway:
Origin of samples
- primarily from primary health care
Transport
- 75 % by car transport to lab.
- 16 % by post
- 9 % collected at lab.
Sample collection
- fasting (not been eating, drinking or smoking since midnight) for triglycerides (at least 12 h fasting) and glucose
- sample material: Serum from Vacutainer SST 6 mL gel sample tubes
- centrifugation: 1300g for 10 min within 2 hours after collection
Measurement system
- Roche Modular
Trueness: The laboratory have tested Roche Modular for all components using NFKK Reference Serum X and CAL and used the Excel spreadsheet for evaluation of bias. The result for CAL is shown together with the distribution(s) for each component:
| Plots | Comments | |
| Clinical chemistry | ||
| Albumin | 40-70 years, 18-40 and >70 years | Brom Cresol Green method. Estimated bias is +1.4 g/L |
| Bilirubin | Sample must be protected against light. | |
| Calcium | Uncorrected and corrected (estimated bias is +0.04 mmol/L). | |
| Carbamide (urea) | Female, male 18-49 years, male >=50 years | Notice the difference in shape of Fürst and NORIP distributions for individuals > 50 years (see also uric acid). |
| Cholesterol | 18-30 years and 30-50 years, >50 years | |
| Creatininium, corr | At the moment (24/9-04) our results is multiplied with 0.88 to match former trueness from Roche. The distributions from Fürst presented here is divided with 0.88 and then subtracted 26 µmol/L to match latest recommendations from Roche to comply with traceability to reference method. | |
| Glucose | Fasting from midnight before sample collection. | |
| HDL-cholesterol | ||
| Iron | No iron medication within at least 24 h before sample collection. | |
| LDL-cholesterol, Friedewalds formula | 18-29 years and 30-49 years, >= 50 years | Friedewalds formula: LDL-chol. = Cholesterol - HDL-chol. - triglycerides/2.2. Not calculated if triglycerides >4.00 mmol/L. |
| LDL-cholesterol, direct method | 18-29 years and 30-49 years, >= 50 years | Roche, enzymatic method without precipitation |
| Magnesium | No or light and brief stasis at sample collection. | |
| Phosphate | Female, male 18-50 years, male >=50 years | No or light and brief stasis at sample collection. |
| Potassium | No or light and brief stasis at sample collection. | |
| Protein | ||
| Sodium | ||
| TIBC | Measured as transferrin, factor 25.1 µmol/g | |
| Triglycerides | Fasting at least 12 h | |
| Urate (uric acid) | Female, male | Notice the difference in shape of Fürst and NORIP distributions for individuals >50 years. See also carbamide (urea). |
| ALP | ||
| ALT | ||
| Amylase | ||
| Amylase, pancreatic | ||
| AST | ||
| CK | Female, male | Sample must be protected against light. Instruction to patient: The pasient should not have performed unusual heavy physical activity, been subject to physiotherapy or have had intramuscular injections the last 3 days before sample collection. |
| Gamma GT | Female, male | |
| LD | No or light and brief stasis at sample collection. | |
| Hematology | ||
| Hb | Sysmex XE-2100 | |
| Erythrocytes | Sysmex XE-2100 | |
| Hematocrit | Sysmex XE-2100 | |
| MCV | Sysmex XE-2100 | |
| MCH | Sysmex XE-2100 | |
| MCHC | Sysmex XE-2100 | |
| Leucocytes | Sysmex XE-2100 | |
| Trombocytes | Sysmex XE-2100 | |
